RiboGrip™ RNase Inhibitor



Description 1 FR:

RiboGrip™ RNase Inhibitor est un inhibiteur de ribonucléase à base de protéines conçu in silico, qui inactive la RNase A. RiboGrip™ inhibe l'activité de la ribonucléase A en se liant de manière non covalente dans un mode non compétitif à un rapport de 1:1. Ce nouvel inhibiteur a une stabilité améliorée à température ambiante sans perte d'activité jusqu'à 1 mois.

Description 2 FR:

Prévention de la dégradation de l'ARN dans les applications suivantes : Synthèse d'ADNc du premier brin RT-PCR RT-qPCR transcription et traduction in vitro

Méta :

Inhibiteur, RNase, ARN, Dégradation

Description 1 EN:

RiboGrip™ RNase Inhibitor is an in silico-designed protein-based ribonuclease inhibitor, which inactivates RNase A. RiboGrip™ inhibits the activity of ribonuclease A by noncovalently binding in a non-competitive mode at a ratio of 1:1. This novel inhibitor has enhanced stability at room temperature with no loss of activity for up to 1 month.

Description 2 EN:

Preventing RNA degradation in the following applications: First-strand cDNA synthesis RT-PCR RT-qPCR in vitro transcription and translation Concentration: 40 U/µl Source: Purified from an E.coli strain that carries an overproducing plasmid containing a RiboGrip™ RNase Inhibitor gene. Storage and dilution buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 7.5, 100 mM KCl, 0.1 mM EDTA, 5 mM DTT and stabilizers. Recommendations for use: RiboGrip RNase Inhibitor™ is used generally at a final reaction concentration of 1 U/μl. Inactivated by heating at 85°C for 5 min. Included in FIREScript® and SOLIScript® cDNA Synthesis Kits and Mixes, and in all SOLIScript® 1-step RT-PCR kits

Meta :

RNase, Inhibitor, RNA, degradation

Categorie: Molecular biology reagents

Réactifs pour la biologie Moléculaire

Sous-categorie : qPCR et RT-qPCR

qPCR and RT-qPCR

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