HOT FIREPol® Multiplex qPCR Mix is optimized for amplifying multiple targets in a single reaction in real-time quantitative PCR assays. The qPCR Mix comprises all the components necessary (except primers, probes, and template) to perform qPCR: HOT FIREPol® DNA Polymerase, optimized buffer components, ultrapure dNTPs, and MgCl2. HOT FIREPol® Multiplex qPCR Mix is optimized for DNA hydrolysis probes based on the 5' flap endonuclease activity. HOT FIREPol® DNA Polymerase is activated by a 10 min incubation step at 95°C. This prevents the extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.
Probe-based qPCR Mix optimized for amplifying multiple targets in a single reaction Probe-based qPCR master mix that has been optimized for highly sensitive and accurate quantification of up to 4 targets in a single reaction. This master mix was developed for TaqMan® probes but is also suitable for other hydrolysis probe types Analyze 1-4 targets in 1 reaction High specificity and sensitivity Robust amplification of GC-rich targets Contains dUTP to prevent cross- contamination when used in combination with UNG Mix Components HOT FIREPol® DNA Polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start 5x Multiplex qPCR buffer with 15 mM MgCl2: 1x PCR solution – 3mM MgCl2 dNTPs including dUTP: the mix allows UNG treatment to prevent carryover contamination from previous runs. IMPORTANT: UNG is not included in the HOT FIREPol® Multiplex qPCR Mix