TransZol lyses cells with guanidine isothiocyanate. In the process of sample lysis, TransZol can maintain the integrity of RNA. After adding RNA Extraction Agent, the solution is divided into a colorless aqueous phase and a pink organic phase. RNA is in the aqueous phase. RNA can be recovered by precipitation with isopropanol. Isopropyl alcohol recovers protein. Suitable for rapid extraction of total RNA from a variety of tissues and cells.
Highlights • High operational safety: RNA Extraction Agent is used instead of chloroform. • Wide range of applications: suitable for small samples (50-100 mg tissue, 5×106 cells), or large samples (≥1 g tissue or ≥107 cells) for human, animal, plant, blood and bacterial tissue extraction both apply. • Fast extraction: the reaction can be completed within an hour. • Visualization of operation: The solution is pink for easy separation of aqueous and organic phases. • High extraction purity: minimal DNA and protein contamination. • RNA lysate: facilitates RNA preservation and reduces inhibition of reverse transcription reactions. Storage at 2-8℃ in dark for one year. Shipping Ice bag 4℃.