RiboGrip™ RNase Inhibitor is an in silico-designed protein-based ribonuclease inhibitor, which inactivates RNase A. RiboGrip™ inhibits the activity of ribonuclease A by noncovalently binding in a non-competitive mode at a ratio of 1:1. This novel inhibitor has enhanced stability at room temperature with no loss of activity for up to 1 month.
Preventing RNA degradation in the following applications: First-strand cDNA synthesis RT-PCR RT-qPCR in vitro transcription and translation Concentration: 40 U/µl Source: Purified from an E.coli strain that carries an overproducing plasmid containing a RiboGrip™ RNase Inhibitor gene. Storage and dilution buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 7.5, 100 mM KCl, 0.1 mM EDTA, 5 mM DTT and stabilizers. Recommendations for use: RiboGrip RNase Inhibitor™ is used generally at a final reaction concentration of 1 U/μl. Inactivated by heating at 85°C for 5 min. Included in FIREScript® and SOLIScript® cDNA Synthesis Kits and Mixes, and in all SOLIScript® 1-step RT-PCR kits