Description SOLIScript® Reverse Transcriptase (RT) is an in-silico designed chimeric RNA‐directed DNA polymerase. SOLIScript® RT can synthesize a complementary DNA strand from ssRNA or ssDNA and is active at higher temperatuures (up to 60°C). SOLIScript® RT is a robust enzyme for RNA detection and has enhanced stability at room temperature with no activity loss for up to 1 month. This RT contains a RNase H domain with reduced activity.
Reagents SOLIScript® Reverse Transcriptase RiboGrip RNase Inhibitor (40 U/μl) 10x RT Reaction Buffer with DTT, 500 mM Tris-HCl pH 8.3, 500 mM KCl, 30 mM MgCl2, 100 mM DTT Oligo (dT) Primer (100 μM) dNTP MIX (20 mM of each) Water, nuclease free